Table 2.
Summary of BN-PAGE analyses for the four different cultures
| Culture | Culture condition before protein extraction | Chlorinated substrate tested on gel slices | Dechlorinating activity detected? | RDases identified in active gel slicesa |
|---|---|---|---|---|
| KB-1 maintained on TCE | Starved for 5 days and then amended with TCE and H2 | TCE | Yes | KB1_VcrA* |
| cis-DCE | Yes | KB1_BvcA* | ||
| trans-DCE | Yes | KB1_GeobRD* | ||
| VC | Yes | KB1_RdhA5 | ||
| 1,2-DCA | Yes | KB1_TceA | ||
| PCE | Yes | |||
| KB-1 maintained on TCE (same culture as above) | Starved for 5 days and then amended with VC and H2 | TCE | Yes | KB1_VcrA* |
| cis-DCE | Yes | KB1_BvcA* | ||
| trans-DCE | Yes | KB1_GeobRD | ||
| VC | Yes | KB1_RdhA5 | ||
| 1,2-DCA | Yes | KB1_TceA | ||
| PCE | Yes | KB1_RdhA1 | ||
| 1,2-DCA KB-1 subculture | Grown exclusively on 1,2-DCA and methanol for >4 years | TCE | Yes | KB1_VcrA* |
| cis-DCE | Yes | KB1_TceA* | ||
| trans-DCE | Yes | KB1_BvcA | ||
| VC | Yes | KB1_RdhA5 | ||
| 1,2-DCA | Yes | |||
| PCE | Yes | |||
| D. mccartyi strain BAV1 | Grown on cis-DCE | TCE | Yes | BvcA |
| cis-DCE | Yes | |||
| trans-DCE | Yes | |||
| 1,1-DCE | Yes | |||
| VC | Yes | |||
| 1,2-DCA | Yes | |||
| PCE | No | |||
| D. mccartyi strain BAV1 | Grown on 1,2-DCA | 1,2-DCA | Yes | BvcA |
a
The identified RDases are listed in order of decreasing peptide hit counts. Accession numbers are provided in Fig. 4. The dominant RDases are indicated by an asterisk after the RDase name.