Detection by PCR of a specific mangotoxin biosynthesis operon sequence in different P. syringae genomospecies 1 strains. (a) Schematic representation of the four primer pairs designed for mbo operon detection in P. syringae strains. (b) Gel electrophoresis showing the specific 692-bp product generated with primers mbo24-For and mbo24-Rev from strains harboring the mbo operon. Lanes correspond to the following: 1, P. syringae pv. syringae 7C67; 2, P. syringae pv. syringae 7F29; 3, P. syringae pv. syringae 8F21; 4, P. syringae pv. syringae 1559-9; 5, P. syringae pv. syringae CECT127; 6, P. syringae pv. syringae CECT4429; 7, P. syringae pv. syringae CFBP3388; 8, P. syringae pv. syringae DAR77787; 9, P. syringae pv. syringae DAR77789; 10, P. syringae pv. syringae EPS17A; 11, P. syringae pv. syringae NCPPB1239; 12, P. syringae pv. syringae Ps-5; 13, P. syringae pv. syringae UMAF0081; 14, P. syringae pv. syringae UMAF0214; 15, P. syringae pv. syringae UMAF0225; 16, P. syringae pv. syringae UMAF1060; 17, P. syringae pv. syringae UMAF6024; 18, P. syringae pv. syringae UMAF6016; 19, P. syringae pv. pisi 1704B; 20, P. syringae pv. pisi HRI203; 21, P. syringae pv. pisi NCPPB1365; 22, P. syringae pv. avellanae ISPaVe011; 23, P. syringae pv. avellanae ISPaVe2056; 24, P. syringae pv. syringae 7D46; 25, 8B48; 26, P. syringae pv. syringae 8C43; 27, P. syringae pv. syringae ITACyL 488; 28, P. syringae pv. syringae ITACyL 521; 29, P. syringae pv. syringae ITACyL 523; 30, P. syringae pv. syringae ITACyL 526; 31, P. syringae pv. syringae ITACyL 529; 32, P. syringae pv. syringae 1444-5; 33, P. syringae pv. syringae FF5; 34, P. syringae pv. syringae EPSMV3; 35, P. syringae pv. syringae UMAF0167; 36, P. syringae pv. tomato DC3000; 37, P. syringae pv. tomato DCT6D1; 38, P. syringae pv. tomato UMAF4007; 39, P. syringae pv. tomato UMAF6018; 40, P. syringae pv. coronafaciens CECT4389; 41, P. syringae pv. phaseolicola 1448A; 42, P. syringae pv. phaseolicola CYL314; 43, P. syringae pv. aptata DSM50252; 44, P. syringae pv. syringae 1507-7; 45, P. syringae pv. syringae B728a; 46, P. syringae pv. syringae CECT4430; 47, P. syringae pv. syringae 0049; 48, P. syringae pv. syringae UMAF0158; 49, P. syringae pv. tabaci ATCC 11528; M, molecular size marker (HyperLadder I; Bioline).