Fig 1.

General scheme of a DNA scaffold system. Enzymes of a desired metabolic pathway are fused with artificial DNA binding domains consisting of 4 ZFPs. The fused enzymes are then bound to their specific target sites on the DNA scaffold to form an artificial enzymatic cascade. The number of enzymes bound to the DNA scaffold, order of enzymes, relative angles of enzymes to each other, and distance between the binding sites for each enzyme can be controlled in a designable manner by simply changing the sequence of binding sites on the DNA scaffold.