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Kinetic parameters for the enzymes used in this studya
| Enzyme | Km (mM) | kcat (1/s) |
Ki (mM) |
|
|---|---|---|---|---|
| Threonine | Homoserine | |||
| HDH | 0.238 ± 0.04 | 27.9 ± 0.45 | 0.1 | |
| HDH-ADB1 | 0.233 ± 0.08 | 28.3 ± 0.21 | 0.09 | |
| HK | 0.112 ± 0.007 | 17.8 ± 0.38 | 1.2 | 4.6 |
| HK-ADB2 | 0.109 ± 0.02 | 17.1 ± 0.32 | 1.08 | 4.4 |
| TS | 0.321 ± 0.03 | 7.48 ± 0.19 | ||
| TS-ADB3 | 0.313 ± 0.05 | 7.23 ± 0.25 | ||
The data shown are the means and standard deviations for three independent experiments.
