Table 2.
Oligosaccharide production following FP hydrolysis by EG1 and its derivativesa
| Protein | Oligosaccharide(s) | Production in μmol/liter (fold increase) or ratio at indicated time |
|||
|---|---|---|---|---|---|
| 0.5 h | 2 h | 8 h | 24 h | ||
| EG1 | G2 | 80.16 (1) | 252.82 (3.15) | 481.48 (6.01) | 529.57 (6.61) |
| G3 | 23.65 (1) | 60.71 (2.57) | 100.10 (4.23) | 117.29 (4.96) | |
| G4 | 6.62 (1) | 19.71 (2.98) | 30.75 (4.65) | 33.65 (5.09) | |
| G2/G3 | 3.39 | 4.16 | 4.81 | 4.52 | |
| CBM-CBM-CD | G2 | 32.25 (1) | 203.01 (6.29) | 280.69 (8.70) | 335.70 (10.41) |
| G3 | 11.18 (1) | 36.46 (3.26) | 60.63 (5.42) | 92.15 (8.24) | |
| G4 | 2.75 (1) | 10.58 (3.85) | 16.34 (5.94) | 22.28 (8.10) | |
| G2/G3 | 2.88 | 5.57 | 4.63 | 3.64 | |
| CBM-CD-CBM | G2 | 32.05 (1) | 71.40 (2.23) | 112.42 (3.51) | 169.76 (5.30) |
| G3 | 11.98 (1) | 27.74 (2.32) | 43.20 (3.61) | 63.28 (5.28) | |
| G4 | 3.29 (1) | 9.27 (2.82) | 13.82 (4.21) | 19.74 (6.01) | |
| G2/G3 | 2.68 | 2.57 | 2.60 | 2.68 | |
| CD | G2 | 59.19 (1) | 150.42 (2.54) | 194.22 (3.28) | 240.61 (4.07) |
| G3 | 19.03 (1) | 53.03 (2.79) | 66.71 (3.51) | 78.47 (4.12) | |
| G4 | 4.37 (1) | 12.59 (2.88) | 12.99 (2.97) | 14.21 (3.25) | |
| G2/G3 | 3.11 | 2.84 | 2.91 | 3.06 | |
a
G2, cellobiose; G3, cellotriose; G4, cellotetraose. Values in parentheses represent the fold increase values derived from the ratio of the molar amounts of oligosaccharide released at the indicated incubation time and at 0.5 h. G2/G3 values represent the fold increases derived from the ratio of the molar amounts of oligosaccharide released by G2 and G3.