Table 2.
Description of samples that yielded positive results of ANME-1 16S rRNA gene amplification
| Sample | Geographical location, site, and vent | Coordinates (depth, m) | Temp (°C) | Total cell concn (cells ml−1)a | PCR result with indicated primer setb (no. of OTUs) |
No. of clones in clonal library | Detection of group (% of clone library): |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | ANME-1AT | ANME-1a | ANME-1b | ANME-1GBa | ||||||
| MC118 | Gulf of Mexico, Mississippi Canyon, Block 118 | 28°51.47′N, 88°29.52′W (880) | 5 | NDc | + (2) | + (1) | + (2) | 24 | − | + (83)d | + (17)d | − |
| FS611 | JFR,e Axial Seamount, Bag City | 45°54.974′N, 129°59.354′W (1,410) | 11.2 | 1.7 × 105 | ND | ND | + (1) | 96 | − | − | − | + (100) |
| FS625 | JFR, Endeavor Segment, Easter Island | 47°56.88′N, 129°5.967′W, (2,150) | 17.8 | 1.3 × 105 | ND | ND | + (2) | 96 | − | − | − | + (100) |
| FS725 | JFR, Endeavor Segment, Boardwalk | 47°58.11′N, 129°5.24′W (2,150) | 16.4 | 7.1 × 104 | ND | ND | + (2) | 96 | + (2) | − | − | + (98) |
| FS448 | Mariana Arc, NW Rota-1, Fault Shrimp | 14°36.06′N, 144°46.62′E (520) | 25 | 3.49 × 105 | ND | ND | + (1) | 96 | − | + (100) | − | − |
| BG410 | Gulf of California, Guaymas Basin, BIG 1 | 27°00.37′N, 111°24.56′W (2,000) | 50–70 | ND | + (4) | ND | ND | 28 | + (7) | − | − | + (93) |
b
Primer sets: 1, ANME-1-25(F)–ANME-1-1406(R); 2, ANME-1-1118(F)–ANME-1-1406(R); 3, ANME-1-25(F)–ARCH-915(R).
c
ND, not determined.
d
Results for the clone library constructed by using the ANME-1-25(F)–ANME-1-1406(R) primer pair.
e
JFR, Juan de Fuca Ridge.