PCO displaying the effect of N deposition on the sets of actinobacterial (a) and fungal (b) functional genes involved in the decomposition of forest floor material. Dissimilarity matrices were constructed from the Bray-Curtis similarity metric based on the presence/absence of all relevant gene variants. For actinobacterial and fungal functional genes, there were significant treatment (P = 0.022,0.013), site (P = 0.001, 0.001), and interaction (P = 0.005,0.001) effects, respectively. Pairwise comparisons with Monte Carlo simulations found significant treatment effects in sites B and C (P < 0.05) for both actinobacteria and fungi. Sites A and D did not have a significant response to treatment. The error bars indicate standard error.