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. 2013 Feb;79(4):1309–1315. doi: 10.1128/AEM.03085-12

Fig 3.

Fig 3

SDS-PAGE analysis of the purification of DsdA[19132] and DsdA[19080] by nickel-affinity chromatography. Lanes: S, protein standard; 1, cell extract of E. coli after expression; 2, unbound proteins; 3, protein fraction obtained after nickel-affinity chromatography; 4, purified protein after removal of imidazole.