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. 2013 Feb;79(4):1183–1190. doi: 10.1128/AEM.02538-12

Table 1.

Spectrophotometric enzyme activity assays

Assay Activitya
Fold purification
Original sampleb Partially purifiedc protein
Hydroxylamine oxidased 0.02 ± 0.006 0.16 ± 0.1 7.2
Hydrazine oxidased 0.09 ± 0.04 0.46 ± 0.1 5.1
Nitrite reductasee 0.052 ± 0.025 0.31 ± 0.1 6.0
a

For all assays, activities are shown as units ± standard deviation (n = 3).

b

Protein concentration, 2.15 mg ml−1.

c

Protein concentration, 0.15 mg ml−1.

d

One unit is defined as (1 μmol MTT/min)/(mg protein).

e

One unit is defined as (1 μmol methyl viologen/min)/(mg protein).