Table 1.
Assay | Activitya |
Fold purification | |
---|---|---|---|
Original sampleb | Partially purifiedc protein | ||
Hydroxylamine oxidased | 0.02 ± 0.006 | 0.16 ± 0.1 | 7.2 |
Hydrazine oxidased | 0.09 ± 0.04 | 0.46 ± 0.1 | 5.1 |
Nitrite reductasee | 0.052 ± 0.025 | 0.31 ± 0.1 | 6.0 |
For all assays, activities are shown as units ± standard deviation (n = 3).
Protein concentration, 2.15 mg ml−1.
Protein concentration, 0.15 mg ml−1.
One unit is defined as (1 μmol MTT/min)/(mg protein).
One unit is defined as (1 μmol methyl viologen/min)/(mg protein).