Table 3.
Production of acetate, ethanol, and biomass by the wild type, bifunctional aldehyde/alcohol dehydrogenase gene deletion mutants, and complemented strains
| Parameter | Value for straina: |
|||||
|---|---|---|---|---|---|---|
| WT | ΔadhE1 | ΔadhE2 | ΔadhE1 ΔadhE2 | ΔadhE1/adhE1 | ΔadhE1 ΔadhE2/adhE1 | |
| Ethanol production (mM) | 28.9 ± 3.4 | 4.7 ± 2.3 | 27.1 ± 0.5 | 4.4 ± 0.6 | 14.7 ± 0.4 | 11.5 ± 1.3 |
| Acetate production (mM) | 38.5 ± 2.3 | 64.4 ± 1.5 | 41.9 ± 3.9 | 63.0 ± 1.8 | 53.4 ± 3.1 | 60.8 ± 1.7 |
| Cell yield (A600) | 1.4 ± 0.1 | 1.3 ± 0.2 | 1.5 ± 0.1 | 1.1 ± 0.0 | 1.2 ± 0.1 | 1.2 ± 0.1 |
| Doubling time (h) | 5.8 ± 0.2 | 8.4 ± 0.4 | 5.7 ± 0.1 | 7.3 ± 0.2 | 8.7 ± 0.5 | 9.5 ± 1.0 |
a
Data are the means ± standard deviations of quadruplicates. WT, wild-type C. ljungdahlii cells; ΔadhE1, adhE1 deletion mutant; ΔadhE2, adhE2 deletion mutant; ΔadhE1 ΔadhE2, adhE1 and adhE2 double deletion mutant; ΔadhE1/adhE1, adhE1 deletion mutant with the adhE1 gene expressed in trans; ΔadhE1 ΔadhE2/adhE1, adhE1 and adhE2 double deletion mutant with the adhE1 gene expressed in trans.