TABLE 2.
Performance of a real-time multiplex PCR assay for the detection of Brucella spp., B. abortus, and B. melitensis
| Strain | PCR identification
|
||
|---|---|---|---|
| Brucella spp. | B. abortus | B. melitensis | |
| B. abortus | 25/25a | 23/25 | 2/25 |
| B. melitensis | 25/25 | 0/25 | 25/25 |
| B. suis | 10/10 | 0/10 | 0/10 |
| B. canis | 1/1 | 0/1 | 0/1 |
| Brucella spp.b | 2/2 | 0/2 | 0/2 |
| Non-Brucella spp.c | 0/59 | 0/59 | 0/59 |
Number of PCR-positive isolates per number of isolates tested.
Human isolates genetically related to marine mammal strains of Brucella.
Acinetobacter baumannii, Actinobacillus actinomycetemcomitans, Afipia broomeae, Afipia genomospecies 4, Agrobacterium radiobacter, Agrobacterium spp., Bartonella bacilliformis, Bartonella henselae, Bartonella quintana, Bordetella bronchiseptica, Burkholderia cepacia, Burkholderia pseudomallei, Capnocytophaga canimorsus, Cardiobacterium hominis, Eikenella corrodens, Enterococcus faecalis, Enterococcus faecium, Escherichia coli O157:H7, Francisella tularensis, Haemophilus aphrophilus, Haemophilus haemolyticus, Haemophilus influenzae, Haemophilus parainfluenzae, Haemophilus paraphrophilus, Kingella kingae, Moraxella catarrhalis, Neisseria meningitidis, Oligella ureolytica, Ochrobactrum anthropi (n = 9), Ochrobactrum spp. (n = 5), Pasteurella aerogenes, Pasteurella multocida, Pseudomonas aeruginosa, Psychrobacter phenylpyruvicus, Ralstonia pickettii, Roseomonas genomospecies 5, Roseomonas gilardii, Roseomonas spp., Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Streptococcus bovis, Streptococcus pneumoniae, Streptococcus pyogenes, Stenotrophomonas maltophilia, Vibrio cholerae, and Yersinia enterocolitica O:9.