Table 2.
Oligonucleotides used in this study
| Primer | Target gene | Sequencea (5′–3′) | Restriction site |
|---|---|---|---|
| Primer1 | SSI1-2 region | CTCTGTCGACTGACCATCACCAATGCTGTG | SalI |
| Primer2 | CTCTGTCGACAAGTTGTCACCGTCTTCGGT | SalI | |
| Primer3 | tpi | GCCCGAATTCATGGCACGTAAGCCAC | EcoRI |
| Primer4 | GCCCGAATTCTTAAGCGACGCTCGCA | EcoRI | |
| Primer5 | ilvNGE | CTCTGCATGCGTTTCCAGATGACCAACCAG | SphI |
| Primer6 | CTCTTCTAGATTAAGCGGTTTCTGCGCGA | XbaI | |
| Primer7 | ilvCTM | CTCTGCATGCGTTTCCAGATGACCAACCAG | SphI |
| Primer8 | CTCTTCTAGATTAAGCGGTTTCTGCGCGA | XbaI | |
| Primer9 | ilvNGECTM | CTCTGCATGCGTTTCCAGATGACCAACCAG | SphI |
| Primer10 | CTCTTCTAGATTAAGCGGTTTCTGCGCGA | XbaI | |
| Primer11 | avtA | CTCTGTCGACAACTTTGGCAGAGCAGCC | SalI |
| Primer12 | CTCTGTCGACTTGTGGATGTGCTCATGGC | SalI | |
| Primer13b | CTCTACTAGTTCTCTTTCCTTGTGTGCTCC | SpeI | |
| Primer14b | CTCTACTAGTAGGCATTGCCACATAATCCC | SpeI |
a
The restriction site used in the cloning procedure is underlined.
b
Primers used for inverse amplification to truncate a middle region of avtA.