Table 4.
Intracellular concentration of metabolic intermediates from glycolysis at 2 h during l-valine production under oxygen deprivation
| Strain | Metabolite concna (mM) |
|||
|---|---|---|---|---|
| DHAP | GAP | BPG | Pyruvate | |
| Val-1 (BNCTMDLD/ΔLP) | 10.72 ± 1.86 | 0.85 ± 0.12 | 0.05 ± 0.00 | Not detected |
| Val-2 (BNCTMDLD/ΔLP_ΔAc) | 6.02 ± 0.71 | 0.44 ± 0.07 | 0.17 ± 0.01 | Not detected |
| Val-3 (BNCTMDLD/ΔLP_+GP) | 8.17 ± 0.51 | 0.45 ± 0.02 | 0.21 ± 0.01 | 0.97 ± 0.09 |
| Val-4 (BNCTMDLD/ΔLP_ΔAc+GP) | 6.84 ± 0.33 | 0.52 ± 0.05 | 0.19 ± 0.02 | 18.03 ± 2.75 |
| Val-5 (BNGECTMDLD/ΔLP_ΔAc+GP) | 8.33 ± 0.38 | 0.62 ± 0.05 | 0.13 ± 0.01 | Not detected |
| Val-6 (BNGECTMDLD/ΔLP_ΔAc+GP_ilvNGE) | 10.77 ± 0.59 | 0.64 ± 0.03 | 0.12 ± 0.03 | Not detected |
| Val-7 (BNGECTMDLD/ΔLP_ΔAc+GP_ilvCTM) | 7.03 ± 1.35 | 0.56 ± 0.07 | 0.19 ± 0.02 | Not detected |
| Val-8 (BNGECTMDLD/ΔLP_ΔAc+GP_ilvNGECTM) | 7.41 ± 0.75 | 0.83 ± 0.14 | 0.17 ± 0.02 | Not detected |
| Val-9 (BNGECTMDLD/ΔLP_ΔAc+GP_ilvNGECTM_ΔAla) | 7.40 ± 0.83 | 0.78 ± 0.10 | 0.23 ± 0.01 | 4.91 ± 0.57 |
a
DHAP, dihydroxyacetone phosphate; GAP, glyceraldehyde-3-phosphate; BPG, 1,3-bisphosphoglycerate. The data represented are from five analytical replicates from one experiment.