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. 2013 Feb;79(4):1126–1133. doi: 10.1128/AEM.02792-12

Fig 4.

Fig 4

PCR analysis with genomic DNA from S. lividans TK24, S. albus, A. utahensis NRRL 12052, or mutants as the template. By use of primers M13-47 (5′-CGCCAGGGTTTTCCCAGTCACGAC-3′) and RV-M (5′-GAGCGGATAACAATTTCACACAGG-3′), the genotypes of the recombinant strains were confirmed by PCR to be well in agreement with their designed genetic patterns, demonstrating that the ΦC31-directed site-specific recombination event had taken place. Lane 1, no product for wild-type A. utahensis; lane 2, 3.3-kb product for DYG2001; lane 3, 4-kb product for DYG2002; lane 4, 6.6-kb product for DYG2003; lane 5, no product for wild-type S. lividans TK24; lane 6, 3.3-kb product for DYG2004; lane 7, 4-kb product for DYG2005; lane 8, 6.6-kb product for DYG2006; lane 9, no product for wild-type S. albus; lane 10, 3.3-kb product for DYG2007; lane 11, 4.0-kb product for DYG2008; lane 12, 6.6-kb product for DYG2003. All PCR products were sequenced to confirm each mutation.