Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| Lactococcus lactis subsp. cremoris MG1363 | Plasmid free | 10 |
| L. lactis NZ9800 | Strain derived from L. lactis MG1363, ΔnisA, non-nisin producer, pepN::nisRK | 24 |
| Lactobacillus plantarum NCIMB8826 | Originally isolated from human saliva | NCIMB |
| L. plantarum NCIMB8826 Int-1 | NCIMB8826 containing nisRK genes stably integrated to the tRNASer locus | 11 |
| Escherichia coli MC1061 | araD139 Δ(ara-leu)7696 lacX74 galV galK hsr-hsm rpsL | Invitrogen |
| Plasmids | ||
| pNZ8148 | Cmr, L. lactis pSH71 replicon | MoBiTech |
| pMEC252 | pNZ8148 carrying Gluc cDNA optimized for L. plantarum codon fused to the L. plantarum Pldh promoter (lactate dehydrogenase) | This study |
| pMEC253 | pNZ8148 carrying Gluc cDNA optimized for L. lactis codon fused to the L. lactis Pusp45 promoter | This study |
| pMEC256 | pNZ8148 carrying CBRluc cDNA optimized for L. plantarum codon fused to Pldh | This study |
| pMEC257 | pNZ8148 carrying CBRluc cDNA optimized for L. lactis codon fused to Pusp45 | This study |
| pNZ8048luxABCDE | pNZ8048 vector carrying the insert luxABCDE fused to the PnisA promoter | 6 |
a
Cmr, resistance to chloramphenicol.