Table 3.
Validation of changes in proteins revealed by MS-based spectral counting consistent with previously published microarray data
| Microarray fold change (transgenic vs wild-type) in 4 month old mice | |||
|---|---|---|---|
| ↑ regulated in transgenic | ↓ regulated in transgenic | ||
| Cathepsin S | 8.4 ± 0.6 | Contactin-1 | −1.6 ± 0.1 |
| Cathepsin B | 2.0 ± 0.2 | Carboxypeptidase E | −1.4 ± 0.1 |
| Cathepsin L1 | 2.1 ± 0.1 | ||
| Peroxiredoxin-6 | 2.5 ± 0.1 | ||
| Complement C4-B | 6.9 ± 0.9 | ||
| Glial fibrillary acidic protein | 4.0 ± 0.6 | ||
| Serine protease inhibitor A3N | 5.4 ± 0.4 | ||
Fold change ± standard deviation
Note: Validation of putative biomarkers from the current proteomics dataset by previously published RNA microarray data.55 Both up and down regulated proteins were consistent with the RNA microarray data.