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. Author manuscript; available in PMC: 2013 Jul 30.
Published in final edited form as: FEBS Lett. 2012 Jun 16;586(16):2287–2293. doi: 10.1016/j.febslet.2012.05.066

Fig. 2.

Fig. 2

β2-Subunit without regions encoded by exon 5 retains α-subunit associating activity. (A) Schematic representations of β2-subunit gene and protein. The exons and the corresponding regions in the protein are represented by the same colors. Dashed boxes correspond to 5′ and 3′ UTR. (B) Schematic of β2-Δ5-3×FLAG and β2-Δ4,5-3×FLAG mutants. (C) HEK293T cells were non-transfected (nt) or transfected with α-subunit alone, or α-subunit together with β2-subunit, β2-Δ5-3×FLAG or β2-Δ4,5-3×FLAG. Proper expression of α-subunit was confirmed with a polyclonal anti-c-Myc antibody (pMYC). Anti-FLAG monoclonal antibody (mFLAG) was used to detect β2-subunit constructs. Both β2 and β2-Δ5-3×FLAG constructs were readily expressed while the β2-Δ4,5-3×FLAG mutant was not detected (N = 3). (D) β2-Δ5-3×FLAG mutant co-IP with the α-subunit similar to the wild type β2 subunit. The IP of α-subunit was carried out with pMYC antibody and β2 subunit mutants were detected with mFLAG antibody (N = 3).