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. Author manuscript; available in PMC: 2013 Jul 30.
Published in final edited form as: FEBS Lett. 2012 Jun 16;586(16):2287–2293. doi: 10.1016/j.febslet.2012.05.066

Fig. 3.

Fig. 3

β2-Subunit without regions encoded by exon 4 retains α-subunit associating activity. (A) β2-Δ4-3×FLAG mutant was expressed in HEK 293T cells and detected with anti-FLAG monoclonal antibody (mFLAG) by Western blot. The α-subunit was detected with a polyclonal anti c-myc antibody (pMYC) (N = 3). (B) β2-Δ4-3×FLAG mutant co-IP with α-subunit similar to the wild type β2 subunit. The IP of α-subunit was carried out with pMYC antibody and β2 subunit mutants were detected with mFLAG antibody by Western blot (N = 3).