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. Author manuscript; available in PMC: 2013 Dec 17.
Published in final edited form as: Free Radic Biol Med. 2012 Oct 23;53(12):10.1016/j.freeradbiomed.2012.10.546. doi: 10.1016/j.freeradbiomed.2012.10.546

Figure 2.

Figure 2

Nox2 is required for VEGF- and H2O2-induced EC Ca2+ influx and migration. A. HAEC were treated with specific siRNA to Nox2 and knockdown was confirmed by qRT-PCR for Nox2 mRNA (n = 3, *** p< 0.001). B. Nox2 was knocked down in HAEC and then migration into a scratch wound over 6 hours was measured in response to either VEGF (50 ng/mL) or H2O2 (1 μM, n = 3, *p < 0.05, **p < 0.01, *** p< 0.001). C. HAEC were treated with siRNA for Nox2 and the maximal increase in intracellular Ca2+ associated with Ca2+ entry upon Ca2+ readdition was measured (n = at least 4, ***p< 0.001). D and E. Representative Ca2+ influx trace in response to VEGF or H2O2 in HAEC in which Nox2 was knocked down.