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Ectopic expression of siRNA-resistant PDK1 rescues motility in siPDK1-treated PCa cells. The left panel shows the quantified results of migration comparing PC-3 cells transfected with siCtrl or siPDK1-1 in combination with an empty vector (Mock) or a siRNA-resistant PDK1-V5 expressing vector at 3, 6, and 12 hours after wounding. Accompanying Western blots are shown on the right, representing expression levels of PDK1, AKT, and p-AKT.
