TABLE 2.

Average C_ts of seven SNP assays with different primer sets on templates consisting of chromosomal DNA of M. tuberculosis^a

Primer set	Match Ct	Mismatch Ct	ΔCt	No. of rejected assays (n = 13)
LP	25.0	32.6	7.6	6
HP	19.6	30.8	11.2	0
LHP	19.6	29.1	9.5	1
ELHP	21.1	32.1	11.0	1

^aSee the text for a description of the isolates used. Match reaction, PCR in which the 3′ end of the primer is complementary to the target. Mismatch reaction, PCR in which the 3′ end of the primer is not complementary to the target. ΔC_t, average of mismatched C_ts − average of matched C_ts.