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. Author manuscript; available in PMC: 2013 Feb 11.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Jul 22;412(1):160–164. doi: 10.1016/j.bbrc.2011.07.066

Fig. 1.

Fig. 1

PTH is not able to increase ATF4 mRNA expression in the absence of endogenous FGF2 in BMSCs in vitro. ATF4 mRNA expression is significantly reduced in Fgf2−/− BMSCs compared to Fgf2+/+ BMSCs at basal level. PTH treatment significantly increased ATF4 mRNA expression in Fgf2+/+ BMSCs as early as 15 min. However, PTH does not increase ATF4 mRNA expression in Fgf2−/− BMSCs at the time points examined. Freshly isolated BMSCs from 3 months old male mice were cultured in αMEM with 10% FBS for 6 days, serum deprived 14 h, then treated with 1 nM PTH or vehicle at the indicated times, followed by total RNA extraction for gene analysis. Data are mean ± SEM for two to three independent experiments.