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. Author manuscript; available in PMC: 2013 Feb 11.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Jul 22;412(1):160–164. doi: 10.1016/j.bbrc.2011.07.066

Fig. 2.

Fig. 2

Anabolic action of PTH on bone formation is impaired in Fgf2−/− mice compared to wild type littermates on a new mix genetic background of black swiss 129Sv/FVB/N. Increased vertebral BMD (A) and BMC (B) by PTH injection is attenuated in Fgf2−/− mice. PTH stimulation of genes important in osteoblast differentiation; type I collagen Col1a1 (C), osteocalcin (D) and Runx2 (E) by PTH is attenuated in tibia of Fgf2−/− mice. 21–23 ms old female mice were injected subcutaneously 5×/week for two weeks with either vehicle or PTH (40 lg/kg BW) as described in materials and methods. Two days after the last injection, BMD and BMC were determined and total RNA was extracted from whole tibia excluding epiphyses for gene analysis by qPCR.