Table 3.
Expression levels and fluorescence intensity of MHC and co-stimulatory molecules on the surface of 5-FU-treated cellsa, b.
| Cell type | Percentage of molecule expression, % | Average fluorescence intensity | ||||||
|---|---|---|---|---|---|---|---|---|
| MHC I | MHC II | CD80 | CD86 | MHC I | MHC II | CD80 | CD86 | |
| CT-26 | ||||||||
| Treated | 92.7 ± 2.76 | 10.0 ± 1.56c | 92.4 ± 7.85 | 9.2 ± 1.84c | 13.6 ± 0.21c | 12.5 ± 0.42d | 4.3 ± 0.14c | 3.5 ± 0.49c |
| Untreated | 99.1 ± 1.27 | 1.5 ± 0.14 | 83.4 ± 6.58 | 3.1 ± 2.47 | 38.9 ± 2.34 | 5.0 ± 0.49 | 2.7 ± 0.35 | 1.8 ± 0.64 |
| TC-1 | ||||||||
| Treated | 92.4 ± 2.69 | 1.6 ± 0.49 | 95.9 ± 4.03 | 5.3 ± 0.10c | 3.0 ± 0.35c | 3.6 ± 0.92c | 10.7 ± 0.99c | 2.9 ± 1.06 |
| Untreated | 97.5 ± 2.47 | 0.4 ± 0.07 | 95.0 ± 1.27 | 1.6 ± 0.49 | 8.2 ± 0.78 | 2.3 ± 0.64 | 5.6 ± 0.14 | 1.8 ± 0.71 |
| 4T-1 | ||||||||
| Treated | 69.7 ± 6.65 | 10.5 ± 1.13d | 11.3 ± 1.27c | 7.8 ± 1.27c | 2.6 ± 0.49c | 5.4 ± 0.49c | 4.4 ± 0.85 | 3.5 ± 1.06 |
| Untreated | 84.4 ± 0.64 | 2.4 ± 1.20 | 2.7 ± 0.71 | 1.8 ± 0.07 | 3.8 ± 0.07 | 3.2 ± 0.64 | 2.7 ± 1.13 | 2.4 ± 0.92 |
Abbreviations: MHC, major histocompatibility complex.
aExperiments were repeated twice with similar results.
bData are expressed as mean ± SD of the two independent experiments.
c, dThe t-test was carried out to determine the difference between the two groups in three experiments: cP < 0.05, dP < 0.01, t-test).