Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 Feb 11.

Published in final edited form as: Transfusion. 2009 Oct 10;50(2):324–333. doi: 10.1111/j.1537-2995.2009.02438.x

Fig. 4 — Reactions of antibodies from Case 1 (Sta), Case 2 (Kno), and Case 3 (Sta) with recombinant mutated GPIIb/IIIa expressed in CHO cells using MACE. Capture of GPIIb/IIIa was achieved with a mixture of MoAbs Tab, AP2, and AP3. Maternal antibody from each case diluted 1:20 reacted with GPIIb/IIIa mutated to contain the appropriate mutation (■) but failed to recognize wild-type GPIIb/IIIa ( ). Anti-HPA-1a recognized both constructs and normal serum (NP) recognized neither. Horizontal lines indicate mean values obtained with normal serum + 3 SD.

Inline graphic — Reactions of antibodies from Case 1 (Sta), Case 2 (Kno), and Case 3 (Sta) with recombinant mutated GPIIb/IIIa expressed in CHO cells using MACE. Capture of GPIIb/IIIa was achieved with a mixture of MoAbs Tab, AP2, and AP3. Maternal antibody from each case diluted 1:20 reacted with GPIIb/IIIa mutated to contain the appropriate mutation (■) but failed to recognize wild-type GPIIb/IIIa ( ). Anti-HPA-1a recognized both constructs and normal serum (NP) recognized neither. Horizontal lines indicate mean values obtained with normal serum + 3 SD.