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. Author manuscript; available in PMC: 2013 Feb 11.
Published in final edited form as: J Microbiol Methods. 2009 Sep 29;79(3):266–271. doi: 10.1016/j.mimet.2009.09.012

Table 2.

Comparison of oral phyla abundance obtained using different classification tools.

Phylum Percentage of total sequences classified
Saliva+throat swab
Saliva (Keijser et al., 2008)
GASTa MG-RASTb RDPc
Firmicutes 61.2 28.6 39.3 40.7
Proteobacteria 29.3 29.9 27.8 21
Actinobacteria 4.9 4.1 2.8 6.3
Fusobacteria 2.9 0.93 1.6 2.9
TM7 1.2 1.2 0.0063 1.9
Bacteroidetes 0.027 0.026 0.021 27.2
BRC1 0.07 0.076 0 0
OP10 0.12 0.040 0 0
Spirochaetes 0.20 0.20 0.064 0.2
Cyanobacteria 0.0007 0.0020 0.0016 0.020
SR1 0.013 0 0.0013 0.014
Acidobacteria 0.0018 0 0 0.049
OP3 0.0006 0 0 0
Unclassified Bacteria 0.023 35 28.5 0.2

NR, not reported.

a

≤30% divergent from their nearest reference sequence.

b

Maximum e-value 10−10 and minimum alignment length of 50 nucleotides.

c

80% confidence cutoff applied at the phylum level.