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. Author manuscript; available in PMC: 2013 Feb 11.
Published in final edited form as: Methods Mol Biol. 2011;756:133–148. doi: 10.1007/978-1-61779-160-4_6

Fig. 1.

Fig. 1

Experiment illustrating the difficulty of accurately measuring ligand efficacy. Concentration-response of VIP(1–28) ( filled circles ) and VIP(2–28) (open circles) stimulated adenylyl cyclase activity in plasma membrane from CHO cells expressing a high (850 ± 50 fmol receptor/mg proteins) or low (100 ± 30 fmol receptor/mg proteins) level of VIP receptor. Adapted from (3).