TABLE 1.

Oligonucleotide primers and detection probes for real-time PCR for the simultaneous detection of E. histolytica, G. lamblia, C. parvum and PhHV-1 (internal control)

Target organism and oligonucleotide	Oligonucleotide sequence	GenBank accession no. or reference
E. histolytica
Ehd-239F	5′-ATTGTCGTGGCATCCTAACTCA-3′	X64142, Z49256
Ehd-88R	5′-GCGGACGGCTCATTATAACA-3′	X64142, Z49256
histolytica-96T	VIC-5′-TCATTGAATGAATTGGCCATTT-3′-nonfluorescent quencher	X64142
G. lamblia
Giardia-80F	5′-GACGGCTCAGGACAACGGTT-3′	M54878
Giardia-127R	5′-TTGCCAGCGGTGTCCG-3′	M54878
Giardia-105T	FAM-5′-CCCGCGGCGGTCCCTGCTAG-3′-black hole quencher 1	M54878
C. parvum
CrF	5′-CGCTTCTCTAGCCTTTCATGA-3′	11
CrR	5′-CTTCACGTGTGTTTGCCAAT-3′	11
Crypto	Texas Red-5′-CCAATCACAGAATCATCAGAATCGACTGGTATC-3′-black hole quencher 2	11
PhHV-1
PhHV-267s	5′-GGGCGAATCACAGATTGAATC-3′	26
PhHV-337as	5′-GCGGTTCCAAACGTACCAA-3′	26
PhHV-305tq	Cy5-5′-TTTTTATGTGTCCGCCACCATCTGGATC-3′-black hole quencher 2	26