ML phylogenetic tree based on the partial S segment nucleotide sequences (nt 378 to 762) of H196 and other DOBV strains, computed with TREE-PUZZLE (Tamura-Nei evolutionary model). The DOBV S segment sequence analyses included the following (accession numbers in parentheses): DOB-PR (AF060018), DOB-TD (AF060023), AP/13Af/99 (AJ410619), Esl/400Af (AY168576), Slo/Af (L41916), DOB-Af1 (AJ251996), DOB-EA (AF060020), DOB-SZ (AF060022), As-1 (AF442622), P-s1223 (AF442623), Saa/160v (AJ009773), Saa/90Aa (AJ009775), Esl/29Aa (AY533118), Esl/81Aa (AY533120), Esl33/Aa (AY533119), Esl/197Aa (AY533121), Esl/862Aa (AJ269550), Slo/9Aa (AJ251999), Kur/53Aa (AJ131673), and H169 (AY533117). HTNV strain 76-118 (M14626) and SEOV strain SR11 (M34881) were used to root the tree. Two DOBV main branches are marked by a light-gray background; the patient-associated sequence is indicated by a dark-gray box and boldface. The values at the tree branches represent the PUZZLE support values.