Skip to main content
. 2004 Mar;42(3):1064–1068. doi: 10.1128/JCM.42.3.1064-1068.2004

FIG. 1.

FIG. 1.

Genotypic analysis by PCR. (A) A1 IS6110 insertion in the dnaA-dnaN intergenic region (14). Lanes 1 to 7, Seattle outbreak isolates. Lane 8, control M. tuberculosis strain H37Ra. A ∼640-bp product is seen in the H37Ra control, and a ∼2,000-bp product is seen in the Seattle outbreak isolates, indicating an IS6110 insertion in the origin of replication. (B) Multiplex PCR for IS6110 insertions in the NTF region (18). Lanes 1 and 2, Beijing family control strains. Lanes 3 to 5, Seattle outbreak isolates. Lanes 6 and 7, non-W-Beijing control strains KY and H37Ra, respectively. Beijing strains are expected to have one IS6110 insertion in the NTF region, resulting in a 223-bp PCR product. W strains, which were not included in this experiment, have a second insertion resulting in a 175-bp product. The 500-bp product is an internal PCR control, and larger bands are nonspecific products, which were seen previously in some strains analyzed under these conditions (18).