Figure 2.
Effect of plasmin on PAR1 receptor activation. Proteolytic activation of PAR1 at 5 minutes was monitored flow cytometrically using antibody SPAN12 to detect intact (i.e., unactivated) receptor. Results were expressed in units of relative fluorescent intensity (RFI), calculated by dividing the mean fluorescent staining intensity obtained with SPAN12 antibody by the staining intensity obtained with a class matched (IgG1) control antibody. Results were expressed as the median ± interquartile range (IQR) from n = 4 experiments. (a) Preformed plasmin (5 U/mL). (b) Plasmin generated in situ from plasminogen. Rest = resting; Pls = plasmin; Apr = aprotinin 200 KIU/mL.