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Effect of plasmin or PAR1 agonist peptide on glial cell detachment. (a) A172 cells were stimulated with plasmin (5 U/mL) for the length of time indicated, and cell detachment was quantified as defined in the Materials and Methods. (b) Effect of PAR1 specific activating peptide SFLLRN or inactive control peptide FSLLRN (both 25 μmoles/L) on cell detachment at 24 hours. Results were expressed as the median ± interquartile range (IQR) from n = 4 experiments.
