Fig. 8.

Selective heterodimerization of bZIP transcription factors after induction by the AAR. A: HepG2 cells were incubated in DMEM (D) or DMEM + 2 mM HisOH (H) for 3, 6 or 12 h and then analyzed for protein-protein interactions by immunoprecipitation (IP) and immunoblotting (IB) with the indicated antibodies against specific members of the ATF, cJUN, or C/EBP families. The blots shown are representative of multiple experiments. B: the coimmunoprecipitation data from multiple experiments are summarized as follows: +, strong association; ±, weak but detectable association, −, little or no interaction. Note that for any pair of antibodies the result may vary depending on the strength of the antibody when it is used for the IP or IB portion of the study.