Figure 5.

Uptake of AS by aged primary murine astroglial cells. Astroglial cells were treated with different AS-forms (full length soluble AS–sAS, fibrillized AS–fAS, and C-terminally truncated AS–tAS). Cells were fixed with 4% paraformaldehyde and immunostained for GFAP (green) and AS (red). A: Both TLR4^+/+ and TLR4^−/− astroglial cells presented incorporated AS in the cytoplasm (scale bar 20 μm). B: Representative three-dimensionally reconstructed confocal images of GFAP-positive astroglia with incorporated sAS are shown (scale bar 10 μm).