TABLE 2.
Virus genes targeted in nested multiplex RT-PCRs to detect the nine respiratory viruses
| Round | Size (bp) of amplicon obtained in the following assay:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Influenza virus
|
RSV and HuMV
|
PIV
|
||||||||
| Controla | Influenza A virus nucleo- capsid | Influenza B virus nucleo- capsid | RSV A nucleo- capsid | RSVB nucleo- capsid | HuMV F protein | PIV-1 hemagglutinin- neuraminidase | PIV-2 hemagglutinin- neuraminidase | PIV-3 hemagglutinin- neuraminidase | PIV-4 phospho- protein | |
| First | 761 | 490 | 722 | 702 | 702 | 526 | 362 | 506 | 477 | 441 |
| Second | 506 | 296 | 402 | 343 | 251 | 454 | 316 | 203 | 109 | 246 |
a
The control amplicon was designed to be larger then than all the virus amplicons in both rounds of the nested PCR.