TABLE 2.

Comparison of the ORF 1b and N gene assays with SARS-CoV RNA^a

Log RNA dilution	ORF 1b assay			N gene assay			ΔCTb
	Mean CT	SD	% CV	Mean CT	SD	% CV
1	18.2	0.17	0.93	16.9	0.17	0.99	1.3
2	22.1	0.75	3.38	20.5	0.31	1.52	1.6
3	25.8	0.18	0.7	24.4	0.66	2.69	1.4
4	31.4	2.57	8.21	27.6	2.18	7.87	3.8
5	33.3	0.7	2.1	31.9	0.19	0.58	1.4
6	37.1	0.1	0.27	36.8	0.23	0.63	0.3

^aEach dilution of SARS-CoV RNA was tested in duplicate in two runs of each assay (n = 4).

^bΔCT = ORF 1b C_T − N gene C_T.