FIG. 1.
Human dental pulp stromal cell (HDPSC) viability and growth on three-dimensional (3D) 45S5 Bioglass® scaffolds. (A) Confocal laser scanning microscope image showing HDPSC viability (arrow–CMFDA) on 45S5 Bioglass scaffolds after 2 weeks of in vitro culture under basal condition. (B, C) scanning electron microscope (SEM) images showing HDPSC spreading and cell bridge formation (arrows) on 45S5 Bioglass scaffolds after in vitro culture under basal conditions for 2 weeks (B) and 4 weeks (C). (D) Alkaline phosphatase (ALP) staining (arrow) indicative of osteogenic differentiation of HDPSC–45S5 Bioglass scaffolds after 4 weeks of culture in basal medium. Color images available online at www.liebertpub.com/tea