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FISH analysis of the alphoidtetO-HAC in HT1080 cells using the telomere probe after several steps of MMCT transfer. FISH analysis was performed using PNA labeled probes for telomeric (red) and tetO-alphoid sequences (green). Panels c and d represent metaphase spreads hybridized with telomeric and tetO-alphoid probes, correspondingly. Panel a represents merged images of panels b, c, and d.
