Table 2. NBD fluorescence lifetimes of WT and D12N AAP in the presence and absence of 2 mM Arg.
Expt | AAP | average τ (ns) | average τ (ns) | lifetime (ns) difference (high Arg-low Arg) |
||
---|---|---|---|---|---|---|
high Arg | chi-square | low Arg | chi-square | |||
1 | WT | 6.6 | 1.5 | 5.3 | 2.0 | 1.3 |
2 | WT | 5.7 | 1.0 | 4.6 | 0.8 | 1.1 |
1 | D12N | 4.9 | 0.3 | 5.7 | 0.4 | −0.8 |
2 | D12N | 5.2 | 1.0 | 5.7 | 1.1 | −0.5 |