Expression profile of mGluRs in human and mouse astrocytes. (A to C) Microarray analysis of the expression of mGluR in (A) adult human cortical astrocytes, (B) adult mouse cortical astrocytes, and (C) adult mouse hippocampal astrocytes. Grm3 is the major mGluR expressed by human cortical astrocytes and mouse cortical and hippocampal astrocytes. (D) qPCR analysis of GLT1+ populations confirmed that GRM3 is highly enriched in human cortical astrocytes. (E) qPCR analysis of GLT1+ cell populations showed that Grm3 also is highly enriched in mouse cortical astrocytes. (F) Expression of Grm3 and Grm5 in mouse hippocampal astrocytes isolated from 1-, 2-, 3-, and 12-week-old mice. Error bars, mean ± SEM; n = 3 biological samples. *P < 0.05, one-way ANOVA, Bonferroni multiple comparisons test.