TABLE 2.
Reactivities of MAbs against CPV-2, CPV-2a, CPV-2b, and the Glu-426 CPV-2 mutants by hemagglutination inhibition
| Isolate | Reactivities of MAbsa
|
Antigenic type, amino acid change | |||
|---|---|---|---|---|---|
| A4E3 | B4A2 | C1D1 | B4E1 | ||
| DP/83 | 160 | 160 | 0 | 1,280 | CPV-2, Asn-426 |
| 188-01 (B) | 640 | 80-160 | 1,280 | 40 | CPV-2a, Asn-426 |
| 108-02 (44) | 640 | 80-160 | 2,560 | 20-40 | CPV-2a, Asn-426 |
| 119-02 (A) | 640 | 80 | 1,280 | 20-40 | CPV-2a, Asn-426 |
| 42-01 | 2,560 | 0 | 2,560 | 20-40 | CPV-2b, Asp-426 |
| 198-02 (A) | 640 | 0 | 1,280 | 0 | CPV-2b, Asp-426 |
| 205-02 | 1,280 | 20 | 1,280 | 10 | CPV-2b, Asp-426 |
| 56-00 | 320 | 0 | 1,280 | 20 | CPV-2?, Glu-426 |
| 136-00 | 1,280-2,560 | 5-10 | 2,560 | 20 | CPV-2?, Glu-426 |
| 157-02 (5) | 320 | 0 | 640-1,280 | 20-40 | CPV-2?, Glu-426 |
| 202-02 (1) | 160 | 0 | 640 | 20 | CPV-2?, Glu-426 |
| 202-02 (1) | 160 | 0 | 640 | 20 | CPV-2?, Glu-426 |
a
Values indicate the reciprocal of the highest dilution of MAb inhibiting eight hemagglutination units of the virus. MAb specificities are as follows: A4E3 for CPV-2, CPV-2a, and CPV-2b; B4A2 for CPV-2 and CPV-2a; C1D1 for CPV-2a and CPV-2b; and B4E1 for CPV-2.