Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 Feb 11.

Published in final edited form as: Cancer Lett. 2011 Apr 14;306(2):205–213. doi: 10.1016/j.canlet.2011.03.008

The depth of maximal mucosal hemoglobin concentrations co-localizes with the increase in MVD.

Fig 2a. Mucosal hemoglobin concentration was measured biophotonically with LEBS. The measures are the differential between AOM-treated and age-matched controls expressed as “effect size” expressed as a percentage. As can be seen, while at all depths there was a positive effect size with AOM (more hemoglobin), the maximal differential appeared to be at ~200-225 microns from tissue surface. As one probes deeper, there is a modest diminution in the AOM versus saline differences in hemoglobin.

Figure 2b: In order to understand the histological correlates for this ~200-225 microns optimal effect size in Hb we analyzed H&E stained sections of the colonic crypts were imaged at 20x magnification using an Olympus BH-2 brightfield microscope equipped with a SPOT camera. Scale bars were added using ImageJ software (NIH) to show various depths of the colonic mucosa. It appears that~200-225 microns from the tissue surface corresponds to the base of the crypts consistent with the hypothesis related to increased mucosal oxygen demand.

The depth of maximal mucosal hemoglobin concentrations co-localizes with the increase in MVD.

Fig 2a. Mucosal hemoglobin concentration was measured biophotonically with LEBS. The measures are the differential between AOM-treated and age-matched controls expressed as “effect size” expressed as a percentage. As can be seen, while at all depths there was a positive effect size with AOM (more hemoglobin), the maximal differential appeared to be at ~200-225 microns from tissue surface. As one probes deeper, there is a modest diminution in the AOM versus saline differences in hemoglobin.

Figure 2b: In order to understand the histological correlates for this ~200-225 microns optimal effect size in Hb we analyzed H&E stained sections of the colonic crypts were imaged at 20x magnification using an Olympus BH-2 brightfield microscope equipped with a SPOT camera. Scale bars were added using ImageJ software (NIH) to show various depths of the colonic mucosa. It appears that~200-225 microns from the tissue surface corresponds to the base of the crypts consistent with the hypothesis related to increased mucosal oxygen demand.