Effect of NGCC on the CT responses in rinse solution. A: representative CT trace obtained while the rat tongue was stimulated with R and then with MSG+Bz+SB solutions containing 60 μM or 100 μM NGCC maintained at room temperature. Arrows show time period when the tongue was superfused with R, R+NGCC, MSG+Bz+SB, or MSG+Bz+SB+NGCC. B: mean normalized tonic CT responses to 300 mM NaCl, 100 mM KCl, 100 mM CaCl2, 500 mM sucrose, 20 mM quinine, and 20 mM HCl in the absence and presence of 2.5 and 50 μM NGCC in 3 rats. *P = 0.0184, **P = 0.0002 (unpaired; n = 3).