. 2012 Dec 5;109(4):1078–1090. doi: 10.1152/jn.00124.2012

Table 1.

Taste stimuli used for CT experiments

Rinse	Composition, mM	Stimuli	Composition, mM
R^*	10 KCl + 10 HEPES	N^*	10 KCl + 10 HEPES + 100 NaCl
R^*	10 KCl + 10 HEPES	N+Bz^*	10 KCl + 10 HEPES + 100 NaCl + 0.005 Bz
R^*	10 KCl + 10 HEPES	N+Bz+IFF compound^*	10 KCl + 10 HEPES + 100 NaCl + 0.005 Bz + IFF compound (0–0.1)
R^*	10 KCl + 10 HEPES	N+SB^*	10 KCl + 10 HEPES + 100 NaCl + 0.001 SB
R^*	10 KCl + 10 HEPES	N+SB+IFF compound^*	10 KCl + 10 HEPES + 100 NaCl + 0.001 SB + IFF compound (0.0025)
R^*	10 KCl + 10 HEPES	N+Bz+SB+IFF compound^*	10 KCl + 10 HEPES + 100 NaCl + 0.005 Bz + 0.001 SB + IFF compound (0–0.1)
R	10 KCl	MSG+Bz+SB	10 KCl + 100 MSG + 0.005 Bz + 0.001 SB
R	10 KCl	MSG+Bz+SB+IMP	10 KCl + 100 MSG + 0.005 Bz + 0.001 SB + 1 IMP
R	10 KCl	MSG+Bz+SB+IFF compound	10 KCl + 100 MSG + 0.005 Bz + 0.001 SB + IFF compound (0–0.1)
R^†	10 KCl + 10 CaCl₂	MSG+Bz+SB+CaCl₂+IFF compound^†	10 KCl + 10 CaCl₂+ 100 MSG + 0.005 Bz + 0.001 SB + IFF compound (0–0.1)
R	10 KCl	Sweet	10 KCl + 500 sucrose
R	10 KCl	Bitter	10 KCl + 10 mM quinine
R	10 KCl	Sour	10 KCl + 20 mM HCl
R	10 KCl	Nonsodium salts	10 KCl + 100 mM KCl
R	10 KCl	Nonsodium salts	10 KCl + 100 mM CaCl₂
R	10 KCl	Control-1	300 NH₄Cl
R	10 KCl	Control-2	300 NaCl

HEPES was used to buffer the pH of rinse and salt stimuli at pH 6.1.

^†

These solutions were used after topical lingual application of 0.15 mM ionomycin for 30 min. Ionomycin was directly dissolved in dimethyl sulfoxide (DMSO). DMSO by itself does not alter CT responses to taste stimuli (Lyall et al. 1999). MSG, monosodium glutamate; IMP, inosine 5′-monophosphate; Bz, benzamil; SB-366791 (SB), N-(3-methoxyphenyl)-4-chlorocinnamide. International Flavors & Fragrances (IFF) compounds N-geranyl cyclopropylcarboxamide, N-geranyl isobutanamide, N-geranyl 2-methylbutanamide, allyl N-geranyl carbamate, and N-cyclopropyl E2,Z6-nonadienamide were used in the concentration range between 0 and 0.1 mM. All IFF compounds used were readily soluble in water.