Table 2. Functional grouping of differentially expressed proteins.
| Protein | Accession no. | 140A-KO | PLN-KO | |
|---|---|---|---|---|
| SR/ER associated proteins | ||||
| Sarcoplasmic/endoplasmic reticulum Ca2+ ATPase | ATA2 MOUSE | 23 | 23.7 | — |
| Cardiac Ca2+ release channel | Q9ERN6 | 5.2 | 2.4 | ▴ |
| Calsequestrin, cardiac muscle isoform | CAQC MOUSE | 21.9 | 14.2 | — |
| Calreticulin | CRTC MOUSE | 11.5 | 14.7 | — |
| FKBP-rapamycin-associated protein | FRAP MOUSE | 1 | 1 | — |
| Cop-coated vesicle membrane protein p24 | P24 MOUSE | 6 | 3 | ▴ |
| Calcium/calmodulin-dependent protein kinase II | Q92991 | 10.8 | 10.8 | — |
| Transitional endoplasmic reticulum ATPase | TERA MOUSE | 8.3 | 2.9 | ▴ |
| SEC22, vesicle trafficking protein-like 1 | Q91VU3 | 3.3 | 0 | ▴ |
| Endoplasmin | ENPL MOUSE | 9.2 | 0 | ▴ |
| Calumenin | CALU MOUSE | 8.3 | 3.2 | ▴ |
| Calnexin | CALX MOUSE | 11.7 | 11.3 | — |
| Intracellular signaling cascade | ||||
| Tyrosine-protein kinase BLK | BLK HUMAN | 2.4 | 0 | ▴ |
| Guanine nucleotide-binding protein, α-11 | GB11 MOUSE | 3.3 | 0 | ▴ |
| Guanine nucleotide-binding protein G, α-2 | GBI2 MOUSE | 6.5 | 0 | ▴ |
| Guanine nucleotide-binding protein β subunit | GBLP HUMAN | 7.6 | 0 | ▴ |
| Guanine nucleotide-binding protein G, α | GBQ MOUSE | 3.4 | 0 | ▴ |
| Myosin-IXa | Q9UNJ2 | 0.2 | 0 | — |
| Multiple PDZ domain protein | Q9Z1K3 | 2 | 2 | — |
| Ras-related protein RAL-A | RALA MOUSE | 9.7 | 0 | ▴ |
| Ras-related protein RAP-1A | RAPA HUMAN | 12.5 | 6.5 | ▴ |
| Transforming protein P21/H-RAS-1 | RASH MOUSE | 5.8 | 0 | ▴ |
| Ras-related protein Rab-10 | RB10 HUMAN | 6 | 0 | ▴ |
| Ras-related protein Rab-1A | RB1A HUMAN | 8.8 | 0 | ▴ |
| Ras-related protein Rab-21 | RB21 MOUSE | 24.5 | 14.3 | ▴ |
| Ras-related protein Rab-5B | RB5B HUMAN | 5.6 | 0 | ▴ |
| Ras-related protein R-Ras | RRAS MOUSE | 5 | 0 | ▴ |
| Superoxide dismutase [Cu-Zn] | SODC MOUSE | 30.1 | 17 | ▴ |
| Cell surface receptor-linked signal transduction | ||||
| Adenylate kinase iscenzyme 1 | KAD1 MOUSE | 26.3 | 4.1 | ▴ |
| Glucagon receptor precursor | GLR HUMAN | 1.7 | 0 | ▴ |
| Integrin β-1 precursor | ITB1 MOUSE | 4.1 | 0 | ▴ |
| Carbohydrate metabolism | ||||
| Fructose-bisphosphate aldolase A | ALFA MOUSE | 7.7 | 3.3 | ▴ |
| α enolase | ENOA MOUSE | 2.5 | 0 | ▴ |
| Phosphoglycerate kinase 1 | PGK1 MOUSE | 8.4 | 0 | ▴ |
| Phosphoglycerate mutase 2 | PMG2 MOUSE | 4.4 | 0 | ▴ |
| Triosephosphate isomerase | TPIS MOUSE | 13.7 | 0 | ▴ |
| Fatty acid regulation | ||||
| Fatty aldehyde dehydrogenase | DHA4 MOUSE | 2.9 | 0 | ▴ |
| Fatty acid-binding protein, heart | FABH MOUSE | 49.2 | 0 | ▴ |
| Long-chain fatty acid transport protein precursor | FATP MOUSE | 2.2 | 2.2 | — |
| Apolipoprotein A-1 precursor | APA1 MOUSE | 26.1 | 12.5 | ▴ |
| Very-low-density lipoprotein receptor precursor | LDVR MOUSE | 2.3 | 0 | ▴ |
| Ion transport | ||||
| Sarcoplasmic/endoplasmic reticulum Ca2+ ATPase | ATA2 MOUSE | 23 | 23.7 | — |
| Sodium/calcium exchanger 1 precursor | NAC1 MOUSE | 5.4 | 5.7 | — |
| ATPase, Na+K+ transporting, α1 polypeptide | Q8VDN2 | 12 | 9.3 | ▴ |
| Cardiac Ca2+ release channel | Q9ERN6 | 5.2 | 2.4 | ▴ |
| Plasma membrane calcium-transporting ATPase 1 | ATB1 HUMAN | 1.1 | 0 | ▴ |
| Inward rectifier potassium channel 2 | IRK2 MOUSE | 5.6 | 2.1 | ▴ |
| Voltage-dependent anion-selective channel | POR1 MOUSE | 50.3 | 66.6 | — |
| Voltage-dependent anion-selective channel | POR2 MOUSE | 34.9 | 25.8 | — |
| Voltage-dependent anion-selective channel | POR3 MOUSE | 33.6 | 30 | — |
| Cytoskeleton | ||||
| Actin, cytoplasmic 1 | ACTB HUMAN | 7.7 | 4.8 | ▴ |
| Desmin | DESM MOUSE | 22.4 | 12.4 | ▴ |
| Ezrin | EZRI MOUSE | 3.1 | 0 | ▴ |
| Similar to ARP1 actin-related protein 1 homolog B | Q8R5C5 | 0 | 6.1 | ▾ |
| Cytovillin 2 | Q9UJU1 | 0 | 10.6 | ▾ |
| β-sarcoglycan | SGCB MOUSE | 13.8 | 0 | ▴ |
| Spectrin α chain, erythrocyte | SPCA MOUSE | 7.1 | 0 | ▴ |
| Spectrin β chain, erythrocyte | SPCB MOUSE | 3.5 | 0 | ▴ |
| Spectrin α chain, brain | SPCN MOUSE | 6.5 | 5.5 | — |
| Spectrin β chain, brain 1 | SPCO MOUSE | 4.1 | 0.9 | ▴ |
| Vesicle-associated membrane protein 2 | VAM2 MOUSE | 14.8 | 0 | ▴ |
| Vesicle-associated membrane protein 3 | VAM3 MOUSE | 16.5 | 0 | ▴ |
| Vimentin | VIME MOUSE | 15.3 | 2.8 | ▴ |
| Class 1 β-tubulin. Tubulin β-5 chain | TBBX HUMAN | 16 | 12.8 | — |
| Developmental processes | ||||
| α-2-HS-glycoprotein precursor | A2HS MOUSE | 0 | 6.1 | ▾ |
| Annexin II | ANX2 HUMAN | 0 | 5.3 | ▾ |
| Myosin light-chain alkali, nonmuscle isoform | MLEN MOUSE | 17.7 | 6.4 | ▴ |
| Myosin light-chain 1, slow-twitch/ventricular | MLEV MOUSE | 0 | 45.1 | ▾ |
| Myosin regulatory light chain 2, ventricular isoform | MLRV MOUSE | 15.2 | 7.3 | ▴ |
| Myosin heavy chain, last skeletal muscle, | MYH3 MOUSE | 14.5 | 0 | ▴ |
Differential regulation of select subsets of functionally related proteins was found in the ventricular membrane fractions of 140A-KO transgenic mice as compared with PLN-KO mutant mice. Protein-abundance ratios are based on the percent peptide coverage obtained in each protein expression and were obtained by averaging three experiments in pooled ventricular tissues. Each protein was classified to a specific annotation category according to the GO schema. For a more complete listing of the proteomic data, see Table 3. ▴, up-regulated, ratio of 140A-KO/PLN-KO ≥ 2; ▾ down-regulated, ratio of 140A-KO/PLN-KO ≤ 2; —, no change.