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. 2004 Feb 12;101(8):2259–2264. doi: 10.1073/pnas.0308762101

Fig. 1.

Fig. 1.

p53 acetylation strongly enhances its DNA binding activity in vitro.(A) Western blot analysis of purified acetylated p53 (lane 3), unacetylated p53 (lane 1), and a preparation of unresolved acetylated and unacetylated p53 (lane 2) proteins with the antiacetylated p53-specific antibody (Bottom), the DO-1 antibody (Middle), or the PAb421 antibody (Top). (B) Schematic representation of the short and long probes in the p21 promoter region used for EMSA. (C) EMSA assay of unacetylated p53 (lanes 2–4 and 9–11) and acetylated p53 (lanes 5–7 and 12–14) with short (lanes 1–7) and long (lanes 8–14) probes. (D and E) The DNA/protein complexes in C were quantitated by phosphorimaging by using imagequant software (Amersham Biosciences). (D) Results with the short probe. (E) Results with the long probe.