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. 2004 Feb 12;101(8):2259–2264. doi: 10.1073/pnas.0308762101

Fig. 4.

Fig. 4.

Acetylation of p53 enhances its DNA binding on endogenous promoters. (A) ChIP assay with equal amounts of immunoprecipitated acetylated and total p53 proteins (lanes 1 and 2). A Western blot with the DO-1 antibody shows the amounts of immunoprecipitated p53 proteins, either by anti-p53 (FL) (lane 4) or by antiacetylated-p53 (lane 5) antibodies, used in the ChIP assays in lanes 1 and 2. Cells were treated by etoposide before the ChIP assay. (B) The resulting DNA amounts in A were quantitated by phosphorimaging by using imagequant software. (C) ChIP assays with anti-p53 (FL) (lane 1) and antiacetylated-p53 (lane 2). Western blot with the DO-1 antibody shows the amounts of immunoprecipitated p53 proteins, either by anti-p53 (FL) (lane 4) or by antiacetylated-p53 (lane 5) antibodies, used in the ChIP assays in lanes 1 and 2. Cells were treated with deacetylase inhibitors TSA and nicotinamide before the ChIP assay. (D) The resulting DNA amounts in C were quantitated by phosphorimaging using imagequant software.