Figure 6. Evaluation of trypsin inhibitor Novartis848 on intrapancreatic trypsin activity and edema formation using mPEG-PL-Cy5.5 probe.

Animals were administered PBS, vehicle, or varying concentration of Novartis848 IP 1 h prior to caerulein induction of pancreatitis (n = 5∼7). Vehicle-1 = vehicle for Novartis848+ SC PBS, Vehicle-2 = vehicle for Novartis848+ SC caerulein. A) Animals were administered the mPEG-PL-Cy5.5 probe to monitor the activity of trypsin in caerulein induced pancreatitis animals. Animals receiving 30 mg/kg of Novartis848 and caerulein showed marked reduction in the signal intensity in the areas of the pancreas compared to vehicle animals. B), C) & D) Pancreas were excised, weighed and fluorescent images were acquired at the end of a 3 h caerulein induced pancreatitis study. B) Fluorescence heat maps of excised pancreas. C) Excised pancreas fluorescence showed results similar to the in vivo imaging study. At 30 mg/kg of Novartis848 the fluorescence of the probe was significantly reduced in comparison to Vehicle-2 animals. (D) The edema ratio. Data represented mean ± SEM.