Skip to main content
. 2013 Feb 11;8(2):e56378. doi: 10.1371/journal.pone.0056378

Figure 5. Comparison of the proliferation of pre-RTEs and naïve T cells in T-DC coculture system.

Figure 5

Purified pre-RTEs (SP4 thymocytes) and naïve CD4+ T cells were labeled with CFSE and cultured with FLDCs at 10:1 ratio. The proliferation and CD44 upregulation were analyzed 4 days later. The fourth column displayed the T-DC coculture in the transwell system. T cells and DCs were separated by 0.4 µm transwell membrane. In the last column, Ly294002 was added to the T-DC coculture at 10 ng/ml. The numbers in the plot indicate the percentage of cells that diluted CFSE. The experiment was repeated for at least 3 times.