Skip to main content
. 2013 Feb 11;8(2):e56378. doi: 10.1371/journal.pone.0056378

Figure 7. Dendritic cells promote the Qa2 upregulation of SP3 pre-RTEs.

Figure 7

Purified SP3 thymocytes were cultured with IL-7 and FLDCs derived from C57BL/6 mice (A, B, E), and Aire -/- mice (C), and MHC class II-/- mice (D) for 4 days. The expression of Qa2 (B–D) and in some cases, Qa2 and CD69 (A) of live T cells were analyzed. (E) SP3 thymocytes upregulate their Qa2 expression in lymphoreplete mice. CFSE-labeled SP3 thymocytes were adoptively transferred into C57BL/6 mice. Qa2 expression was analyzed in CFSE+ T cells in the lymph nodes and spleen at 2 and 4 days post-transfer. The numbers show the percentage of Qa2+ cells. The experiments have been repeated for 3 times.